Synthetic Seed Production and Germplasm Conservation of Enicostema axillare (Lam.) raynal ssp. littoralis (Blume) raynal Using Nodal and Root Explants

In the present study, synthetic seed production of medicinally important plant Enicostema axillare through nodal and root explants were attempted. plantlets germinated seeds using in vitro explants. Synthetic prepared from 3% Na+ 100mm calcium chloride was optimum concentration for uniform compact synseed formation. Alginate beads containing derived successfully entrapped with sodium alginate half-MS, quarter-MS, full-strength MS, double-distilled water 100 mM CaCl2 solution. full strength MS medium exhibited highest number multiple shoots on benzyl amino purine (BAP) 2 mgl-1 kinetin (KIN) 0.5 combination GA3. The capsulated produced 15.0±1.87 at a high rate (87%) BAP KIN combinations GA3 shoot length 6.24 ±1.03 cm along 28.4±5.50. stored 18ºC found to be favorable temperature preservation seeds, it increased germination frequency. ¼ preserved up 80 days more without losing ability (80%). fully developed hardened vermiculite (100%) survival 71%. This protocol could used conservation germplasm long-term storage E. axillare.